Proteomics

The MRI Proteomics Center combines extensive experience and state-of the-art instrumentation for customized method development and sample analysis. Advanced MS instrumentation, high performance robotics, and expertise in multiple analytical approaches provide exceptional insight and flexibility in analytical proteomics analysis. Ongoing programs include proteome profiling for direct analysis of intact tissue samples, biofluids, and other complex protein mixtures as well as protein separation, identification, and characterization.

General proteomics capabilities include:

• Protein and peptide separation
• 1D and 2D gel electrophoresis
• Gel imaging and analysis
• Sample processing (in gel or in solution digestion, protein/peptide fractionation)
• Protein identification
• Intact mass determination (small molecule, peptide, protein)
• Post-translational modification characterization

Contact:

Sarah Schwartz, Ph.D.
Senior Chemist,
Proteomics Center
(816) 360-2481

Contact:

Thomas Grant,
Ph.D.
Director, Business Development
(816) 360-5328

If your proteomics needs extend beyond these traditional services, MRI can provide a comprehensive custom approach to your problem. The MRI Proteomics Center staff have expertise in sample preparation, analysis, and data processing and interpretation.

Specialized proteomics services include:

• Biomarker discovery (from intact tissues, biological fluids, sample extracts)
• Customized assay development
• Method evaluation and validation
• Sample evaluation requiring specialized facilities
• GLP and GMP services are available

Competitive pricing is available.

MS signal profiling and principal component analysis (PCA) of biomarker patterns to segregate sample populations

Instrumentation

The Proteomics Center is well-equipped for sample processing and characterization. Instrumentation includes:

• Applied Biosystems Voyager-DE STR MALDI TOF mass spectrometer
• Applied Biosystems 4000 Q-TRAP mass spectrometer
• Applied Biosystems API 4000 mass spectrometer
• Waters Quattro II and Quattro LC system
• Dionex Ultimate 3000 Nano and Capillary LC system
• Genomic Solutions ProPic and ProPrep Workstations
  

Specialized Facilities

MRI can accommodate samples that require handling conditions more restrictive than conventional facilities. MRI Biosafety Level (BSL) -2 and BSL-3 laboratories are registered with the Centers for Disease Control and Prevention to handle select biological agents and toxins. These BSL laboratories hold several permits with the USDA and follow the Department of Defense program for handling these dangerous pathogens. The MRI Proteomics Facility is a BSL-2 level laboratory equipped to conduct microbiology studies, handle potential pathogens, and further process samples prepared in other BSL laboratories.

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